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goat antirage (R&D Systems)

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R&D Systems goat antirage
Goat Antirage, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+antirage/Human%2FMouse%2FRat+RAGE%2FAGER+Antibody/pm39639058-244-111-116
Average 94 stars, based on 66 article reviews

goat antirage - by Bioz Stars, 2026-09

94/100 stars

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Article Title: Cell competition drives bronchiolization and pulmonary fibrosis.
Article Snippet: Mice containing LSLrtTA3 were placed on doxycycline containing chow (rodent diet with 625mg/kg doxycycline; Harlan Teklad TD.09761) on the day of bleomycin. .. Immunofluorescent staining was performed with the following primary antibodies: rabbit anti-Merlin (NF2; 1:250; clone A-19; sc-331; RRID:AB_2298548; Santa Cruz Biotechnology), rabbit anti-phosporylated-Mst1(Thr183)/2(Thr180) (1:200; 3681; RRID:AB_330269; Cell Signaling Technologies), rabbit anti-phosphorylated Yap (Ser127) (1:200; 4911; RRID:AB_2218913; Cell Signaling Technologies), goat anti-Scgb1a1 (1:200; clone T-18; sc-9772; RRID:AB_2238819; Santa Cruz Biotechnology Inc.), goat anti-Sox9 (1:500; AF3075; RRID:AB_2194160; R&D Systems), mouse anti-Keratin 17 (1:50; clone Ks17.E3; sc-101461; RRID:AB_2234376; Santa Cruz Biotechnology, Inc.), chicken anti-GFP (1:500; GFP-1020; RRID:AB_10000240; Aves Labs Inc.), rabbit antiKeratin 5 (1:200; clone EP1601Y; MA5-14473; RRID:AB_10979451; Thermo Fisher Scientific), chicken anti-Keratin 5 (1:500; 905901; RRID:AB_2565054; BioLegend), rabbit anti-SFTPC (1:200; WRAB-9337; RRID:AB_2335890; Seven hills bioreagents), rat anti-RAGE (1:500; Clone 175410; MAB1179; RRID:AB_2289349; R&D Systems), goat antiRAGE (1:500; AF1145; RRID:AB_354628; R&D Systems), rat anti-Keratin 8 (1:100; TROMA-I; RRID:AB_531826; Developmental Studies Hybridoma Bank), Syrian hamster anti-podoplanin (PDPN, T1a; 1:500; 8.1.1; RRID:AB_531893; Developmental Studies Hybridoma Bank), rabbit anti-mucin 5b (Muc5b; 1:250; cloneH-300; sc-20119; RRID:AB_2282256; Santa Cruz Biotechnology Inc.), mouse anti-alpha actin (smooth muscle actin (SMA), Acta2; 1:500; Clone 1A4; sc-32251; RRID:AB_262054; Santa Cruz Biotechnology Inc.), rabbit anti-myc (1:200; clone Y69, ab32072; RRID:AB_731658; Abcam), rabbit anti-human Myc (1:200; clone C-19; sc-788; RRID:AB_631277), rabbit anti-collagen I (1:500; ab34710; RRID:AB_731684; Abcam), mouse anti–beta-tubulin (1:500; clone 3F3-G2; LMAB-3F3; RRID:AB_451728; Seven Hills Bioreagents), and rabbit anti-p63 (1:500; clone poly6190; 619002; RRID: AB_2207170; BioLegend). .. After deparaffinization, slides were rehydrated through a series of decreasing ethanol concentrations, antigen unmasked by either microwaving in citrate-based antigen unmasking solution (Vector Labs, H-3300) or by incubating sections with proteinase K (7.5μg/ml) (Invitrogen, 25530-049) for 7min at 37 °C.

Incubation:

Article Title: A 1-42-RAGE Interaction Disrupts Tight Junctions of the Blood-Brain Barrier Via Ca2+-Calcineurin Signaling
Article Snippet: Serial 30- m-thick coronal tissue sections were cut using a freezing microtome (Leica). .. Free-floating sections were incubated with the following primary antibodies: biotin-labeled 4G8 (1: 700; Covance), rabbit anti-GLUT-1 (1:1000; Millipore), and goat antiRAGE (1:20; R & D Systems) overnight at 4°C. .. After washes in PBS, the sections were incubated with the following secondary antibodies: Alexa Fluor 488-conjugated streptavidin (1:1000; Invitrogen), goat anti-rabbit Alexa 594 (1:1000; Invitrogen), and biotinylated anti-goat IgG antibody (1:500; Vector Laboratories) for 1 h and then donkey anti-goat Alexa 594 for 1 h for RAGE detection at RT.

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